The US company brings together 25 compounds, a monthly senolytic cycle and a tracking app in a system that aims to address in a coordinated way the main biological mechanisms associated with aging.
The longevity industry continues to explore formulas to intervene on the biological mechanisms that accompany aging. In this context, the company TimeWarp presents its Longevity Protocol, a program that combines daily supplementation, a monthly intervention with senolytics and digital tools to also integrate certain lifestyle habits.
Its proposal, called Protocol 01, starts from a premise: to approach aging as a complex biological phenomenon, in which different cellular and physiological processes maintain a close relationship. The company presents it as the first commercial protocol designed to cover the 14 hallmarks of aging, or biological characteristics of aging, according to the scientific classification updated in 2025.
The development of this formula would have required approximately 40,000 evaluations using a proprietary bioinformatic optimization model. Scientists and specialists linked to institutions such as MIT, Stanford, Harvard, UC Irvine and the Buck Institute have participated in the project.
From nine to fourteen mechanisms of aging
The concept of hallmarks of aging constitutes one of the most widely used reference frameworks in contemporary longevity research. Its first formulation, published in 2013, identified nine biological processes related to aging. A 2023 update expanded the classification to twelve.
In April 2025, a new paper published in the journal Cell, associated with researchers Carlos López-Otín and Guido Kroemer, expanded the model to fourteen characteristics.
Among the additions are alterations of the extracellular matrix, the structure that provides support and organization to tissues, and psychosocial isolation, which includes phenomena such as loneliness and social disconnection.
This expansion has important implications for the design of longevity interventions. Some mechanisms can be addressed through specific compounds, while others require interventions related to behavior, environment and social relationships.
The doctor David Furman, professor at the Buck Institute and director of the Stanford 1000 Immunomes Project, who led the scientific review of the formula, considers this integrated perspective essential. In his view, the mechanisms of aging are deeply interconnected and treating them as independent targets does not adequately reflect the complexity of the process.
Twenty-five compounds and a monthly intervention with senolytics
The Longevity Protocol is organized into four capsule formulas: Core, Energize and Protect, intended for daily consumption, and Cleanse, reserved for a monthly senolytic intervention.
The first three bring together the compounds that the company has selected to act on twelve of the fourteen mechanisms of aging. The fourth incorporates a two-day cycle per month targeting senescent cells, popularly known as zombie cells.
These are cells that have stopped dividing but remain active in the body and can contribute to inflammatory processes and alterations of the tissue environment. Senolytics are substances investigated for their ability to selectively eliminate some of these cells.
TimeWarp has opted for intermittent administration, rather than daily exposure. The company justifies this decision based on the approach used in various human studies on senolytics, in which short administration cycles have been studied.
Dr. Deepti Agarwal, a triple-board-certified medical specialist and medical director of Precision Medical Care, in Chicago, reviewed the protocol from a clinical perspective. According to her, research with senolytics tends to use administrations concentrated in short periods, followed by intervals during which the body is expected to regain its balance.
The TimeWarp program incorporates two consecutive days of monthly administration. The company maintains that this interval responds to the dynamics of senescent cell accumulation, although the optimal frequency of these cycles remains a question that requires specific clinical research.
The app incorporates the two mechanisms that cannot be resolved with supplements
The last two mechanisms of the fourteen-element classification pose a different challenge: social isolation and alterations of the extracellular matrix.
Kristen Fox, founder of TimeWarp, acknowledges that no combination of supplements can by itself compensate for a sedentary life or the absence of social relationships. For this reason, the company has incorporated a behavioral dimension through its app.
The digital tool fulfills three main functions. The first is to facilitate adherence to the program, through recording daily doses and scheduling the monthly senolytic cycles.
The second consists of proposing habits backed by research on health and longevity: exposure to natural light during the morning, walks after waking up, strength training, breathing exercises, periods of focus without digital interruptions and routines to improve sleep preparation.
Two habits have special relevance within the company's approach: maintaining a daily practice of social connection and engaging in physical activity, including resistance training, to contribute to maintaining the structure and function of tissues.
The third function allows users to consult an estimate of biological age in relation to chronological age and the rate of aging, using the user's analytical results or a habits assessment carried out within the app itself.
The app is included in the protocol, with no additional subscription fee.
How the 25 compounds were selected
The development of the formula began with an initial selection of 240 substances. TimeWarp subjected them to its own bioinformatics model that analyzes 162 biological submodules and thirteen mechanistic pathways located upstream of the fourteen hallmarks of aging.
The process generated around 40,000 evaluations of the interaction between compounds and submodules. To establish the ranking, the company gave greater weight to clinical studies in humans, followed by mechanistic research and, finally, results obtained in animal models.
The result was a selection of 25 substances: fifteen considered by the company as its core longevity compounds and another ten incorporated for their potential to reinforce or complement the action of the former.
The final composition was defined taking into account various practical and scientific constraints: dosage, capsule capacity, bioavailability, safety and interactions between ingredients.
The doctor Vittorio Sebastiano, professor of Biological Chemistry at UC Irvine and adjunct professor at Stanford School of Medicine, highlights that the approach starts from the biological mechanisms of aging and then selects the ingredients, rather than building a formula from a list of substances popular on the market.
Synergies between ingredients: beyond the sum of components
One of the aspects TimeWarp highlights about its formulation is the design of combinations between ingredients that participate in related metabolic pathways.
One example is the pairing of NMN and apigenin. NMN is a precursor of NAD+, a molecule essential for numerous cellular processes, including energy metabolism and cellular maintenance and repair functions.
With aging, NAD+ levels can decline. Among the factors involved is the activity of CD38, an enzyme that consumes this molecule. Apigenin has been studied for its ability to inhibit CD38, hence the interest in combining both compounds.
Kristen Fox summarizes the logic behind this association with a comparison: administering NMN or NR without paying attention to CD38 activity would be like filling a tank that has a leak.
The formula applies similar reasoning to other nutrients. Calcium from Ca-AKG is combined with vitamin D3, which promotes its absorption, vitamin K2 MK-7, linked to calcium metabolism and bone health, and magnesium, necessary for various activation and utilization processes of vitamin D.
Methyl group metabolism has also been taken into account. The conversion of NMN generates nicotinamide, whose metabolism and elimination involve methylation processes. To support these pathways, the formula incorporates 5-MTHF, an active form of folate, methylcobalamin, and vitamin B6 in the form of P5P.
The company claims to have designed more than twenty interactions of this type among the different ingredients.
For Sebastiano, aging cannot be addressed through a single molecule. Its biological complexity requires considering multiple metabolic and cellular pathways simultaneously.
Bioavailability: the importance of the form used
The amount of a compound present in a capsule does not necessarily determine the amount the body can absorb and utilize. Bioavailability therefore constitutes a relevant variable in the design of any supplementation formulation.
TimeWarp claims to have selected different forms of the ingredients with the aim of improving their absorption or availability.
Among the changes introduced are the use of dihydroberberine instead of conventional berberine, pterostilbene instead of resveratrol, and isoquercetin instead of standard quercetin. It also uses liposomal formulations for ingredients such as curcumin, EGCG, and fisetin, and uses active forms of B-group vitamins.
Sulforaphane, a compound present in cruciferous vegetables such as broccoli, receives particular treatment. The protocol incorporates delayed-release capsules with glucoraphanin, the enzyme myrosinase, and vitamin C. The purpose is to facilitate the precursor reaching the intestine accompanied by the elements necessary for its conversion into sulforaphane.
In total, fourteen of the twenty-five compounds use active, enhanced, or liposomal forms, according to information provided by the company.
Dr. Agarwal notes that a compound with poor absorption may have limited biological utility, even if its mechanisms of action appear promising in experimental studies.
In the same vein, Stefanie Morgan, vice president of Research and Applied Sciences at AgelessRx, emphasizes that bioavailability was considered a central variable from the early stages of development.
A multidisciplinary team and manufacturing controls
Five specialists with expertise in immunology, epigenetics, clinical medicine, translational research, and bioinformatics participated in the design of the protocol.
David Furman led the scientific review of the formula. Vittorio Sebastiano, co-founder of Turn Biotechnologies, has more than fifty scientific publications, including research in journals such as Nature, Science y Cell. Deepti Agarwal contributes her clinical experience and her connection to the Next Generation Longevity conference in Osaka.
Stefanie Morgan participated as principal investigator in PEARL, one of the first one-year randomized trials of rapamycin in healthy adults. For her part, Danielle Orozco Cosio holds a PhD in Neuroscience from MIT, where she also studied Molecular Biology, and co-founded a bioinformatics platform before joining MyDose AI.
According to TimeWarp, manufacturing is carried out in facilities certified under cGMP standards and registered with the FDA. The company also states that products are subjected to external testing for heavy metals and microbiological contamination in a laboratory accredited under ISO 17025.
Adherence as part of the design
One of TimeWarp's commercial arguments focuses on a common problem with supplementation programs: the difficulty of maintaining them over time.
Longevity routines often combine numerous products, doses, and schedules from different manufacturers. The company considers that this complexity reduces adherence and compromises potential results. Its response consists of integrating daily capsules, the monthly senolytics cycle, and habit tracking into a single experience.
Fox summarizes this philosophy by noting that consistency is an indispensable condition for any health intervention: an isolated exercise session does not transform physical fitness, just as taking a supplement occasionally is unlikely to produce the expected effects.
The company calls this architecture an operating system for aging: the formulas constitute the biological component and the app, the digital and behavioral support.
A proposal that will need to be evaluated with clinical evidence
TimeWarp presents Protocol 01 as a new generation of longevity programs, built from an integrated vision of aging mechanisms. Its combination of 25 compounds, intermittent senolytic administration, and habit tracking reflects an industry trend toward more structured and personalized protocols.
The company maintains that thirteen of its fifteen main ingredients have been studied in randomized human trials and that eight compounds have human data related to aging markers, including telomere length and immune cell age.
However, it is important to distinguish between the available evidence on individual ingredients and the clinical demonstration that a specific combination produces benefits in the people who consume it. Results obtained with a substance at certain doses, populations or experimental conditions cannot be automatically transferred to a complete formula.
Nor does the theoretical coverage of the fourteen mechanisms of aging demonstrate that the protocol acts effectively on all of them. In particular, the integration of social habits and physical activity within an app represents a behavioral support strategy, not a pharmacological or nutritional intervention on those mechanisms.
The relevant question will be to verify whether continued use of the system produces measurable improvements in health, physical function, metabolic parameters, inflammation or quality of life, and whether those results justify its cost and complexity.
Protocol 01 will be available from November 10, 2026 through the TimeWarp website, with included access to its iOS and Android app.
More information: www.timewarp.com
